Tamar Bates had 29 points and five steals to help Missouri beat Hunter Dickinson and No. 1 Kansas 76-67 on Sunday.
Mark Mitchell scored 17 points in Missouri's first win over Kansas since a 74-71 victory on Feb. 4, 2012.
Missouri fans rushed the court after the final buzzer, something Tigers coach Dennis Gates was encouraging.
"I just think they deserve that moment," said Gates.
Anthony Robinson II had 11 points and five steals for the Tigers (8-1), and Josh Gray grabbed a team-leading 10 rebounds.
Dickinson had 19 points and 14 rebounds, but he also committed seven turnovers. The Jayhawks (7-2) have lost two straight on the road after falling 76-63 against Creighton on Wednesday night.
Missouri opened a 57-33 lead with 14:15 remaining on a jump shot from Tony Perkins. But the Jayhawks outscored the Tigers 30-8 over the next 11:55.
Bates scored 18 to help Missouri to a 39-25 lead at the break. The Tigers then opened the second half with a 16-6 run.
Takeaways
Kansas: Missouri's size and defensive pressure were too much for the Jayhawks, who are 5-5 at Mizzou Arena since it opened in 2004.
Missouri: Improved to 4-5 against Kansas when the Jayhawks are ranked No. 1 in The Associated Press poll.
Key moment
Mitchell hit a 3-pointer to lift Missouri to a 68-63 lead with 2:03 remaining. Gray then hit a pair of free throws and Bates converted a layup to make it 72-63 with 1:16 to play.
Key stat
Kansas committed 22 turnovers, including 14 in the first half.
Up next
Kansas: Hosts NC State on Saturday.
Missouri: Hosts Long Island on Saturday.
The Associated Press contributed to this report.