India's campaign in the mixed team archery event came to an end after Deepika Kumari and Pravin Jadhav lost 2-6 to top-seeded South Korea in the quarterfinals on Saturday.
They had earlier recorded a comeback win over Chinese Taipei to enter the quarterfinals.
World No. 1 Deepika failed to notch a single perfect 10 from eight arrows while Olympic debutant Jadhav scored a six in the decisive fourth set after drilling in three perfect 10s at the Yumenoshima Park.
The Indians failed to shoot even a single 10 in the first set as the Korean duo of An San and Kim Je Deok took the first set 35-32 in a low-scoring affair.
Jadhav picked up tempo in the second set with two 10s, but Deepika has scores of 8 and 9 as they lost the second set 37-38.
Required to win the third set to keep their hopes alive, the Indians shot three 9s and one 8. An San faltered with an 8 on the final arrow to hand India the set.
Needing another win in the fourth set to take the match to a fifth set, Jadhav started off with a 6 that virtually ended their campaign.
In their first match, India trailed 1-3 after they lost the first set 35-36 and split points with a 38-38 second set. They then needed to win the third set to stay alive. Deepika and Jadhav rose to the occasion, shooting four 10s. The Chinese Taipei duo of Lin Chia-En and Tang Chih-Chun could only shoot a combined 35 in response, levelling the match. The Indians started relatively poorly in the fourth set, with a 9 and an 8, but two 10s ensured they edged their opponents 37-36 to take the match 5-3.
South Korea earlier beat Bangladesh 6-0, in the round of 16 of the mixed team event, which is making its Olympics debut at Tokyo 2020.
Following poor scores in the men's ranking round on Friday, India were forced to break the combination of Atanu Das and Deepika, the first Indian couple to take part in the same discipline in an Olympic Games.
Das finished in 35th place, three points and four places behind Jadhav, as the Indian coaches preferred the Olympic debutant, breaking the mixed team pair that won the Paris World Cup last month and pairing Deepika with Jadhav for the first time in an international competition.